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Hcf101 | High chlorophyll fluorescence phenotype protein

345 €

AS06 163 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana | cellular [compartment marker] of plastid stroma

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Item No:
AS06 163

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product information
Background

Hcf101 is a novel protein required for photosystem I biogenesis. Hcf101-1 is a high-chlorophyll-fluorescence hcf) Arabidopsis thaliana mutant that lacks photosystem I. In green tissue the level of this protein is stimulated by light and the protein has not been detected in roots. It has been localized in chloroplast stroma.

Immunogen

recombinant Arabidopsis thaliana Hcf101, amino acid residues 206-532 Q6STH5, At3g24430

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunolocalization (IL)
Related products

AS03 037 | rabbit anti-RbcL stroma marker antibody

Plant protein extraction buffer

Secondary antibodies

Additional information

cellular [compartment marker] of plastid stroma

application information
Recommended dilution

1:3000 with standard ECL (WB), 1: 3000 (IL)

Expected | apparent MW

57 | 50.5 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Arabidopsis thaliana

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Immunolocalization has been done on isolated plastids, followed by separation into stroma and thylakoids

Selected references Hu et al. (2017). The SUFBC2 D Complex is Required for the Biogenesis of All Major Classes of Plastid Fe-S Proteins. Plant J. 2017 Jan 19. doi: 10.1111/tpj.13483.
Bigeard et al. (2014). Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana. Proteomics. 2014 May 31. doi: 10.1002/pmic.201400072.


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