PsaF | PSI-F subunit of photosystem I
AS06 104 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, H. vulgare, N. tabaccum, O. sativa, S. oleracea
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1:1000 with standard ECL (WB)
|Expected | apparent MW||
24 kDa | 17 kDa for Arabidopsis thaliana
|Confirmed reactivity||Arabidopsis thaliana, Briopsis corticulans, Hordeum vulgare (very weak), Nicotiana tabaccum, Oryza sativa, Spinacia oleracea|
Populus trichocarpa, Ricinus communis, Physcomitrella patens, Micromonas sp.
|Not reactive in||
Chlamydomonas reinhardtii, Synechococcus PCC 7942
|Selected references||Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.|
2 µg of total leaf protein of Arabidopsis thaliana (1) and Hordeum vulgare (2) and total cellular protein of Chlamydomonas reinhardtii (3) and Synechococcus PCC 7942 (4) isolated with PEB (AS08 300) were separated on 4-12% Nupage Bis-Tris gels in in MES running buffer (Invitrogen) at 200V for 35 minutes. Proteins were transferred for 80 minutes at 30V to a PVDF membrane pre-wetted in methanol and equilibrated in 1X transfer buffer. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) and probedwith anti-PsaF (AS06 104, 1:1000) and secondary HRP-conjugated goat anti-rabbit antibody (1:50 000, Abcam) for 1 hr in TBS-T containing 2% ECL Advance blocking reagent (GE Healthcare). Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signals was detected after 30 s using ECL Advance detection reagent (GE Healthcare) according to the manufacturers instructions and a CCD imager (FluorSMax, Bio-Rad).
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