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PsaK | PSI-K subunit of photosystem I

323 €

AS04 049 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, H. vulgare, N. tabacum, S. oleracea

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AS04 049

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product information
Background

PsaK is a subunit of photosystem I. It has a role in organizing the peripheral light-harvesting complexes on the core antenna of photosystem I. Alternative names: photosystem I subunit X, PSI-K

Immunogen

fusion protein between DHFR and the mature part of PsaK from Arabidopsis thaliana Q9SUI5, At1g30380

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

antibody collection to PSI proteins

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1:2000- 1: 5000 with standard ECL (WB)

Expected | apparent MW

8.5 kDa

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Spinacia oleracea
Predicted reactivity

n.a.

Not reactive in

Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942

Additional information

not available at the moment

Selected references Bock (2012). The plastid genome-encodedYcf4 protein functions as a non-essential assembly factor for photosystem I in higher plants. Plant Physiol. ahead of print.

application example

2 µg of total protein from (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300),(2) Horderum vulgare leaf extracted with PEB (AS08 300), (3) Chlamydomonas reinhardtii total cell extracted with PEB (AS08 300), (4) Synechococcus sp. 7942 total cell extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:20 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

 

Western blot detection using anti-PsaK antibodies



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