PsbA | D1 protein of PSII, DE-loop
AS10 704 | clonality: polyclonal | host: rabbit | reactivity: A.thaliana, Ch. reinhardii, H. vulgare, M. truncatula, S. vulgaris, S. oleracea, P. patens, P. vulgaris, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803
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1 : 10 000 on thylakoid fraction (WB)
|Expected | apparent MW||
38 | 28-30 kDa
|Confirmed reactivity||Arabidopsis thaliana, Chlamydomonas reinhardii, Hordeum vulgare, Medicago truncatula Physcomitrella patens, Pisum sativum, Silene vulgaris, Sinapsis alba, Spinacia oleracea, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803|
dicots including: Cucumis sativus, Glycine max, Nicotiana tabacum, Ricinus communis, Vitis vinifera, monocots including: Oryza sativa, Zea mays, trees: Populus balsamifera
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
antibody is recognizing a 23 kDa fragment in spinach and Arabidopsis thylakoids
for usage on total cell extracts the dilution needs to be determined experimentally
|Selected references||Kale et al. (2017). Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):2988-2993. doi: 10.1073/pnas.1618922114.
Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. BMC Plant Biol. 2016 Sep 2;16(1):191. doi: 10.1186/s12870-016-0883-4.
Kowalewska et al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015.
Karlsson et al. (2015). The Arabidopsis thylakoid transporter PHT4;1 influences phosphate availability for ATP synthesis and plant growth. Plant J. 2015 Aug 8. doi: 10.1111/tpj.12962.
Malnoë et al. (2014). Thylakoid FtsH Protease Contributes to Photosystem II and Cytochrome b6f Remodeling in Chlamydomonas reinhardtii under Stress Conditions. Plant Cell, Jan 21.
Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.
Block et al. (2013). Functional Modeling Identifies Paralogous Solanesyl Diphosphate Synthases that Assemble the Side Chain of Plastoquinone-9 in Plastids. J Biol Chem. Aug 2.
Spetea et al. (1999). GTP bound to chloroplast thylakoid membranes is required for light-induced, multienzyme degradation of the photosystem II D1 protein. PNAS 96: 6547-6552.
Thylakoid membranes from Arabidopsis (0.05-0.75 µg of Chl) were separated on 14%AA+ 6M urea gels and blotted 1h to PVDF. Blots were blocked immediately following transfer in 5% milk solution for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 20 000 o.n. at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (donkey anti-rabbit IgG horse radish peroxidase conjugated, from GE-Healthcare) diluted to 1:5 000 . The blots were washed as above and developed for 5 min with ECL-Plus detection reagent according to the manufacturers instructions. Exposure time was 1 min in CCD camera Fuji4000.
Courtesy Professor Cornelia Spetea-Wiklund, University of Ghotenburg, Sweden
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