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PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII

345 €

AS05 092  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, H. vulgare, N. tabacum, P. sativum, Z. mays

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AS05 092

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product information
Background

The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include  33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II,  OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.

Immunogen

N-terminually located peptide chosen from Arabidopsis thaliana PsbO1 At5g66570 and 2 proteins At3g50820

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Related products

AS06 142-33 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII

AS06 167 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII

AS08 305 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII

AS06 142-16 | anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII

Plant protein extraction buffer

Secondary antibodies

Additional information

Loading based on 50-100 ng of chlorophyll is enough to obtain good signal with this antibody

application information
Recommended dilution

1: 1000 with ECL (WB)

Expected | apparent MW

33 kDa

Confirmed reactivity

Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Pisum sativum, Sinapsis alba, Zea mays

Predicted reactivity

dicots including Brassica oleracea, Pisum sativum, Vitis viniferaPopulus tremula, Picea sitchensis

Not reactive in

Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942

Additional information

Good signal is obtained with this antibody with a load from 0.5 chlorophyll µg/well.

Selected references
Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. Mazur et al. BMC Plant Biology (2016) 16:191.
Albanese et al.(2016). Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy. Photosynth Res. 2016 Jan 9.
Hu et al. (2015). Site-specific Nitrosoproteomic Identification of Endogenously S-Nitrosylated Proteins in Arabidopsis. Plant Physiol. 2015 Feb 19. pii: pp.00026.2015.
Casanova-Sáez et al. (2014). Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development. J Exp Bot. 2014 Mar 24.
Albus
et al. (2012). LCAA, a novel factor required for Mg protoporphyrin monomethylester cyclase accumulation and feedback-control of aminolevulinic acid biosynthesis in tobacco. Plant Physiol. Oct 19.

application example

2 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Horderum vulgare leaf ), (3) Chlamydomonas reinhardtii total cell , (4) Synechococcus sp. 7942 total cell were all extracted with PEB (AS08 300) and separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). 

 

  western blot detection using anti-PsbO antibody

 


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