ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII

345 €

AS06 142-23  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana , P. banksiana. , S.oleracea, Ch. reinhardtii

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
38 st
Delivery:
Shipping:
Item No:
AS06 142-23

Info: Product suggestions Add review
product information
Background

PSII reaction centre components are  generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site          of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

Immunogen

native, purified 23 kDa protein from Spinacia oleracea

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 260 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS05 092 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII

AS06 142-33 | anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII

AS06 167 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII

AS08 305 | anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII

AS06 142-16 | anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1:2000 - 1: 5000  with standard ECL (WB)

Expected | apparent MW

28 | 23 kDa

Confirmed reactivity

Arabidopsis thaliana , Hordeum vulgare, Pinus banksiana, Spinacia oleracea, Chlamydomonas reinhardti

Predicted reactivity

dicots including: Pisum sativum, Solanum lycopersicum, monocots including: Oryza sativa, trees including: Populus balsamifera, Pinus monticola

Not reactive in

Synechococcus sp. PCC 7942

Additional information

Load per well on cell extract of Pinus banksiana (Jack Pine) was 7 µg.

This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).

Selected references

Wang et al. (2008).  Beta-lactone probes identify a papain-like peptide ligase in Arabidopsis thaliana. Nat Chem Biol. 4: 557-563.


application example

2 µg of total protein from (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300), (2) Hordeum vulgare leaf extracted with PEB (AS08 300), (3) Chlamydomonas reinhardtii total cell extracted with PEB (AS08 300), (4) Synechococcus sp. 7942 total cell extracted with PEB (AS08 300), (5) Anabaena sp. total cell extracted with PEB (AS08 300) were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

 

western blot using anti-PsbP antibodies
 




||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at support@agrisera.com