PsbS | 22 kDa Lhc-like PSII protein (rabbit)
AS09 533 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, F. bidentis, H. vulgare, N. tabacum, O. sativa, P. abies, S. oleracea, Z. mays
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1 : 2000 with ECL (WB)
|Expected | apparent MW||
28 | 22 kDa for Arabidopsis thaliana
|Confirmed reactivity||Arabidopsis thaliana, Cytisus cantabricus (Wilk.) Rchb. F, Flaveria bidentis, Hieracium pilosella L., Hordeum vulgare, Lasallia hispanica, Nicotiana tabacum, Oryza sativa, Picea abies, Ricinus communis, Spinacia oleracea, Syntrichia muralis (Hedw.) Raab, Zea mays|
dicots including: Cucumis sativus, Medicago truncatula, Solanum lycopersicum, Vitis vinifera, monocots including: Oryza sativa, trees: Picea sitchensis, Pinus radiata, Pinus taeda, Populus balsamifera, moss: Physcomitrella patens, algae: Chlamydomonas reinhardtii
|Not reactive in||
No confirmed exceptions from predicted reactivity known in the moment
|Selected references||Míguez et al. (2017). Diversity of winter photoinhibitory responses: A case study in co-occurring lichens, mosses, herbs and woody plants from subalpine environments. Physiol Plant. 2017 Feb 14. doi: 10.1111/ppl.12551.
Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22.
Poudyal et al. (2016). Production of superoxide from photosystem II-light harvesting complex II supercomplex in STN8 kinase knock-out rice mutants under photoinhibitory illumination. J Photochem Photobiol B. 2016 Sep;162:240-7. doi: 10.1016/j.jphotobiol.2016.06.050.
Ishikawa et al. (2016). NDH-Mediated Cyclic Electron Flow Around Photosystem I is Crucial for C4 Photosynthesis. Plant Cell Physiol. 2016 Aug 6. pii: pcw127. [Epub ahead of print]
Pavlovič et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.
Karlsson et al. (2015). The Arabidopsis thylakoid transporter PHT4;1 influences phosphate availability for ATP synthesis and plant growth. Plant J. 2015 Aug 8. doi: 10.1111/tpj.12962.
Dahal et al. (2015). Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink. New Phytol. 2015 May 29. doi: 10.1111/nph.13479.
Belgio et al. (2015). Light harvesting superstructures of green plant chloroplasts lacking photosystems. Plant Cell Environ. 2015 Mar 4. doi: 10.1111/pce.12528.
Lintala et al. (2013). Arabidopsis tic62 trol mutant lacking thylakoid bound ferredoxin-NADP+ oxidoreductase shows distinct metabolic phenotype. Mol Plant Sep 16.
Zienkiewicz et al. (2013).Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plan Physiol Biochem. March 16.
Albus et al. (2012). LCAA, a novel factor required for Mg protoporphyrin monomethylester cyclase accumulation and feedback-control of aminolevulinic acid biosynthesis in tobacco. Plant Physiol. Oct 19.
Application example 1
5 µg of total extract from (1) Arabidopsis thaliana leaf, (2) Spinacia oleracea (3) Hordeum vulgare (4) Zea mays extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.
Application example 2
Courtesy of Dr. Laura Roy, University of Amsterdam, The Netherlands
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