PsbS | 22 kDa Lhc-like PSII protein
AS03 032 | clonality: polyclonal | host: hen | reactivity: A. thaliana, A. sativa, B. napus, H. vulgare, O. sativa, P. sylvestris, P. tremula, S. oleracea
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1: 2000 - 1 : 4000 (WB)
|Expected | apparent MW||
28 | 22 kDa for Arabidopsis thaliana
|Confirmed reactivity||Arabidopsis thaliana, Avena sativa, Brassica napus, Hordeum vulgare, Oryza sativa, Pinus sylvestris, Populus tremula, Spinacia oleracea|
dicots and monocots, conifers
|Not reactive in||
Chlamydomonas reinhardtii, Chlorella sp.
to be added when available
|Selected references||Hubbart et al. (2012). The photoprotective protein PsbS exerts control over CO2 assimilation rate in fluctuating light in rice. The Plant J. March 2012.|
15 µg of Arabidopsis thaliana thylakoids from (1) PsbS-overexpressing line, (2) PsbS-deficient npq4-line, and (3) wt (col) together with (4) total leaf protein from Arabidopsis thaliana wt extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PsbS (AS03 032, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (300s, standard sensitivity). Technical note(s): (a) This IgY shows reactivity to both markers loaded in lane M (MagicMark and NovexSharp, both Invitrogen), for comparison a marker lane from same filter (probed seperately with a IgG-antiserum) is shown to the right. (b) the IgY reacts with 2 bands in leafs and thylakoids that both are absent in the PsbS-deficient npq4 line and therefore both might represent PsbS-forms with a difference in molecular weight of ~1 kDa.
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