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PsbY | Small subunit Y of PSII

345 €

AS06 114 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

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AS06 114

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product information
Background Alternative names: psbY-A1, L-AME, L-arginine.metabolizing enzyme.
Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana PsbY sequence, UniProt:O49347, TAIR: At1g67740
Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution

For reconstitution add 200 ĩl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products

collection of antibodies to photosystem II

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1: 1000 (WB)
Expected | apparent MW

4.7 and 4.9 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Glycine soja , Medicago truncatula, Morus notabilis, Oryza sativa, Populus trichocarpa, Ricinus communi, Solanum chacoense, Spinacia oleracea, Theobroma cacao , Zea mays, Zostera marina
Not reactive in

Cyanobacteria

Additional information
Selected references Von Sydow et al. (2016). The PsbY protein of Arabidopsis Photosystem II is important for the redox control of cytochrome b559. Biochim Biophys Acta. 2016 May 21. pii: S0005-2728(16)30536-9. doi: 10.1016/j.bbabio.2016.05.004.

Application example

Western blot using anti-PsbY antibodies

1 µg of total protein from Arabidopsis thaliana PSII enriched membranes in BBY storage buffer (20 mM MES-NaOH pH 6.3, 400 mM sorbitol, 5 mM MgCl2, 10 mM CaCl2, and 15 mM NaCl) denatured with Laemmli sample buffer at 70C for 5 min were separated on 16,5 % Tris-Tricine, 6 M Urea SDS-PAGE with a 10 % spacer gel, and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 2 % low fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:10 000 in TBS-T for 1h at RT with agitation. The blot was washed as above, the last wash in distilled water, and developed for 2 min with Western Bright ECL from Advansta. Exposure time was 60 seconds.

Courtesy of Lotta von Sydow, KBC, Umeå, Sweden

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