AOX1 | Alternative oxidase 1

345 €

AS06 152  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii  |  cellular [compartment marker] of mitochondrial inner membrane


14 st
Item No:
AS06 152

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product information

The mitochondrial AOX (alternative oxidase) of the unicellular green alga Chlamydomonas reinhardtii is encoded by two different genes, the Aox1 and Aox2. The  alternative respiratory pathway is comprised of a single homodimeric protein – AOX – and functions as a mechanism to decrease the formation of reactive oxygen species (ROS) produced during respiratory electron transport.  Alternative oxidase expression is  influenced by different stress stimuli.


whole presumed mature AOX1 protein from from Chlamydomonas reinhardtii O65000 fused to GST

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS04 054 | anti-plant alternative oxidase 1 and 2 polyclonal antibody

AS10 699 | AOX | alternative oxidase monoclonal antibody

Collection of antibodies to Chlamydomonas proteins

Algal protein extraction buffer

Secondary antibodies

Additional information

cellular [compartment marker] of Chlamydomonas reinhardtii mitochondrial inner membrane

application information
Recommended dilution

1: 10 000 with alkaline phosphatase (WB)

Expected | apparent MW

36 | 36 kDa (Chlamydomonas reainhardii)

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Ostreococcus lucimarinus, Aspergilus niger


Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references Zalutskaya et al. (2015). The Chlamydomonas reinhardtii alternative oxidase 1 is regulated by heat stress. Plant Physiol Biochem. 2015 Dec;97:229-34. doi: 10.1016/j.plaphy.2015.10.014.
Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.
Inman (2013). Characterization of the Alternative Oxidase from the Psychrophilic Green Alga Chlamydomonas sp. UWO241. Thesis.
Bohne et al. (2013). Reciprocal Regulation of Protein Synthesis and Carbon Metabolism for Thylakoid Membrane Biogenesis. PLOS, Open Access.

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