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Beta-CA1, beta-CA2 | carbonic anhydrase | mitochondrial | Chlamydomonas

345 €

AS11 1737   | clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii | marker of mitochondria for low carbon dioxide grown cells

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AS11 1737

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product information
Background

Beta-CA1, beta-CA2 is a low-CO2-induced  mitochondrial carbonic anhydrase. that catalyze the reversible interconversion of carbon dioxide and water to bicarbonate and protons. Synonymes: MtCA.

Immunogen

recombinant Chlamydomonas reinhardtii mitochondrial CA, as described in Villand et al. 1997. Accession number Q39590 and Q39589

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunofluorescence (IF)
Related products

other antibodies against carbonic anhydrase

Algal protein extraction buffer

Secondary antibodies

Additional information

antibody is recognizing both isoforms, beta- CA1 and beta-CA2 and can be used as mitochondrial marker for low carbon dioxide grown cells of Chlamydomonas reinhardtii.

application information
Recommended dilution

1 : 1000 with standard ECL (WB), 1: 200 (IF)

Expected | apparent MW

23.7 | 21-22 kDa

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Chlamydomonas reinhardtii
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

to be added when available

Selected references Muranaka et al. (2015). TEF30 interacts with photosystem II monomers and is involved in the repair of photodamaged photosystem II in Chlamydomonas reinhardtii. Plant Physiol. 2015 Dec 7. pii: pp.01458.2015.
Tirumani et al. (2014). Regulation of CCM genes in Chlamydomonas reinhardtii during conditions of light-dark cycles in synchronous cultures. Plant Mol Biol. 2014 Mar 4.
Renberg et al. (2010). A Metabolomic Approach to Study Major Metabolite Changes during Acclimation to Limiting CO2 in Chlamydomonas reinhardtii. Plant physiol. 154: 187-196.


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