ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

GLDH | Galactono-1,4 lactone dehydrogenase

345 €

AS06 182  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:  H. vulgare, G. max,  A. sativa, Helianthus annuus, Z.mays

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
16 st
Delivery:
Shipping:
Item No:
AS06 182

Info: Product suggestions Add review
product information
Background

Galactono-1,4-lactone dehydrogenase (GLDH) is the enzyme which catalyses last step of ascorbic acid (AA)  synthesis in the mitochondria of plant cells. Synonyme: GaLDH

Immunogen

Recombinant C-terminal of Zea mays GLDH, UniProt: C0HFL3

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

Collection of antibodies to stress proteins

Collection of antibodies to mitochondrial proteins

Additional information

Total IgG concentration is 6.8 μg/μl

application information
Recommended dilution

1: 5000 with standard ECL (WB)

Expected | apparent MW

68 kDa

Confirmed reactivity

Avena sativa, Glycine max, Hordeum vulgare, Helianthus annuus, Zea mays

Predicted reactivity

Arabidopsis thaliana

Not reactive in

No confirmed exceptions from predicted reactivity known in the moment

Additional information

Mitochondrial, membrane or meristematic fractions were shown to be richer in GLDH expression

Selected references
Schimmeyer et al. (2016). L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis. Plant Mol Biol. 2016 Jan;90(1-2):117-26. doi: 10.1007/s11103-015-0400-4. Epub 2015 Oct 31.
Ostaszewska-Bugajska et al. (2016). Changes in the OXPHOS system in leaf and root mitochondria of Arabidopsis thaliana subjected to long-term sulphur deficiency. Acta Physiologiae Plantarum 38:141.
Bartoli et al. (2005). Ascorbate content in wheat leaves is not determined by maximal L-galactono-1, 4-lactone dehydrogenase (GalLDH) activity under drought stress. Plant Cell Environ 28:1073-1081.


||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at support@agrisera.com