ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

GDC-H | H protein of glycine decarboxylase complex (GDC)

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS05 074  |  clonality: polyclonal  |  host: rabbit  |   reactivity: A. thaliana, S. oleracea, T. aestivum, V. faba  |  cellular [compartment marker] of mitochondrial matrix

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
43 st
Delivery:
Shipping:
Item No:
AS05 074

Info: Product suggestions Read reviews
product information
Background

The Glycine decarboxylase complex (GDC) is abundant in mitochondria of C3 leaves and functions in photorespiratory carbon recovery. GDC  enzyme can account for up to 50% of matrix protein, and is responsible for the most prominent metabolic activity in the mitochondria of illuminated leaves, photorespiration. GDC is a multienzyme complex composed of four component enzymes, the P-, H-, T-, and L-proteins and is responsible for the conversion of glycine produced in the peroxisome to serine in the mitochondria during photorespiratory cycle. The H-protein plays a key role as a mobile substrate that commutes between the other subunits, allowing its lipoic acid “arm” to visit the active sites of the other three components.

Immunogen

purified GDC-H protein from Spinacia oleracea

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS06 203A | anti- Idh | isocytrate dehydrogenase rabbit antibody, marker of mitochondrial matrix

AS07 212 | anti-VDAC1 marker antibody for mitochondrial outer membrane

Plant protein extraction buffer

Secondary antibodies

Additional information

cellular [compartment marker] of mitochondrial matrix

application information
Recommended dilution

1:5 000 with standard ECL (WB)

Expected | apparent MW

16 kDa

Confirmed reactivity Arabidopsis thaliana, Spinacia oleracea, Triticum aestivum, Vicia faba
Predicted reactivity

n.a.

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

this antibody can be used on total cell extract of Arabidopsis thaliana

Selected references Bancel et al. (2015). Proteomic Approach to Identify Nuclear Proteins in Wheat Grain. J Proteome Res. 2015 Sep 8.
Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544.
Córdoba-Cañero et al. (2011). Arabidopsis ARP endonuclease functions in a branched base excision DNA repair pathway completed by LIG1. The Plant J in print

application example

 

15 µg of total protein from  Arabidopsis thaliana leaf extract has been loaded per lane. Primary antibody has been used in 1: 5000 dilution using standard ECL.

 

western blot detection using anti-GDC-H antibody
 

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com