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NDPK | Nucleaoside diphosphate kinase

265 €
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AS08 302  |  clonality: polyclonal  | host: rabbit  | reactivity:A.thalina,P.sativum

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AS08 302

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Background

Nucleoside diphosphate kinase protein (EC=2.7.4.6) is catalysing the transfer of a g-phosphate group from adenosine triphosphate (ATP) to a cognate nucleoside diphosphate. This contributes to balancing of the nucleoside pool. NDPK enzymes are present in most subcellular compartments of the eukaryotic cell.

Immunogen

KLH-conjugated synthetic peptide derived from Pisum sativum NDPK, UniProt: Q9SP13. Peptide is conserved in Arabidopsis thaliana NDPKIII, UniProt:O49203 and NDPK IV, UniProt: Q8LAH8, but is not conserved in NDPK1.

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products
Additional information
application information
Recommended dilution

1: 5000 (WB)

Expected | apparent MW

25 kDa

Confirmed reactivity Arabidopsis thaliana, Pisum sativum
Predicted reactivity

dicots including: Brassica campestris, Spinacia oleracea, Vitis vinifera, monocots indlucing: Oryza sativa

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

1: 5000 dilution in a western blot was used for 15 µg of protein per lane

Selected references Hammargren et al. (2007). On the phylogeny, expression and targeting of plant nucleoside diphosphate kinases. Physiol Plant.  129: 79–89. 


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