C-YFP | C-terminal of YFP
AS11 1775 | Clonality: polyclonal | Host: rabbit | Reactivity:C-terminal of YFP
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1 : 10 000 (WB)
|Expected | apparent MW|
C-YFP tagged proteins from Arabidopsis thaliana
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
To be added when available, antibody released in May 2016.
Sample preparation and immunoblot analysis were carried out as described in Karnik et al., Plant Cell 2015, March 2015 vol. 27 no. 3 675-694. For immunoblot analysis of plant tissues,leaves were excised and ﬂash frozen in liquid N2. Frozen tissue was ground in equal volumes (w/v) of homogenization buffer containing 500 mM sucrose, 10% glycerol, 20 mM EDTA,20m MEGTA, ProteaseInhibitor(Roche),10mMascorbicacid,5mM DTT, and 50 mM Tris-HCl, pH 7.4, and centrifuged at 13,000g and 4°C for 30 min to pellet debris. Supernatant was diluted 1:1 in 23 Laemmli buffer containing 2.5% 2-mercaptoethanol, heated to 95°C for 10 min, and separated by SDS-PAGE on a 12% Acrylamide gel. Ponceau S-stained Rubisco bands were used as loading standards for plant samples. Membrane type: Cellulose Nitrate (GE Healthcare) Blocking reagent: GE Healthcare Wash buffer: Tris Buffered Saline, 0.5% Tween Exposure time: 10 – 20 seconds.
Courtesy of Dr. Rucha Karnik, University of Glasgow, UK
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