C-YFP | C-terminal of YFP
AS11 1775 | Clonality: polyclonal | Host: rabbit | Reactivity:C-terminal of YFP
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|Recommended dilution||1 : 10 000 (WB)|
|Expected | apparent MW|
|Confirmed reactivity||C-YFP tagged proteins from Arabidopsis thaliana|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
To be added when available, antibody released in May 2016.
Sample preparation and immunoblot analysis were carried out as described in Karnik et al., Plant Cell 2015, March 2015 vol. 27 no. 3 675-694. For immunoblot analysis of plant tissues,leaves were excised and ﬂash frozen in liquid N2. Frozen tissue was ground in equal volumes (w/v) of homogenization buffer containing 500 mM sucrose, 10% glycerol, 20 mM EDTA,20m MEGTA, ProteaseInhibitor(Roche),10mMascorbicacid,5mM DTT, and 50 mM Tris-HCl, pH 7.4, and centrifuged at 13,000g and 4°C for 30 min to pellet debris. Supernatant was diluted 1:1 in 23 Laemmli buffer containing 2.5% 2-mercaptoethanol, heated to 95°C for 10 min, and separated by SDS-PAGE on a 12% Acrylamide gel. Ponceau S-stained Rubisco bands were used as loading standards for plant samples. Membrane type: Cellulose Nitrate (GE Healthcare) Blocking reagent: GE Healthcare Wash buffer: Tris Buffered Saline, 0.5% Tween Exposure time: 10 – 20 seconds.
Courtesy of Dr. Rucha Karnik, University of Glasgow, UK
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