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PntA (Slr1239) | Pyridine nucleotide transhydrogenase alpha-subunit

265 €
Buy 2 items of this product for 198 €/each
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AS15 2910 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. PCC 6803 

PRODUCT INFORMATION IN PDF

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AS15 2910

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product information
Background PntA (Slr1239) (Pyridine nucleotide transhydrogenase alpha-subunit) is an integral mambrane protein complex which participates in the regulation of ion of NAD(P)+:NAD(P)H redox homeostasis. Functional enzyme is a dimer of PntA and PntB.
Immunogen KLH-conjugated peptide derived from Synechocystis sp. PCC6803 NAD(P) transhydrogenase alpha subunit sequence, UniProt: A0A068N3D0

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum, pH 7.4.
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products AS15 2911 | Anti-PntB (slr1434) | Pyridine nucleotide transhydrogenase beta-subunit, rabbit antibodies

other antibodies to proteins involved in photosynthetic electron transfer

Secondary antibodies

Additional information
application information
Recommended dilution 1: 3000 (WB)
Expected | apparent MW 56 kDa
Confirmed reactivity Synechocystis sp. PCC6803
Predicted reactivity Bacillus subtilis, Cyanothece sp. PCC 7822, Desulfobulbaceae bacterium BRH_c16a, Elusimicrobia bacterium, Fischerella sp. JSC-11, Hapalosiphon sp. MRB220, Magnetococcus marinus, Moorea producens JHB , Pleurocapsa sp. PCC 7327, Stanieria cyanosphaera
Not reactive in algae
Additional information
Selected references Kämäräinen et al. (2017). Pyridine nucleotide transhydrogenase PntAB is essential for optimal growth and photosynthetic integrity under low-light mixotrophic conditions in Synechocystis sp. PCC 6803. New Phytol. 2017 Apr;214(1):194-204. doi: 10.1111/nph.14353.

Application example

Western blot using anti-PntA antibodies


25 µg of total proteins from Synechocystis sp. PCC 6803 (WT) and the ΔpntA mutant extracted with buffer containing 50 mM HEPES-NaOH, pH 7.5, 30 mM CaCl2, 800 mM sorbitol and 1mM 6-aminohexanoid acid and denatured with 2xLaemli buffer at +4 °C for O/N. Proteins were separated on 12 % SDS-PAGE containing 6 M urea and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk for 1h at room temperature (RT) with agitation. After that blot was washed with TTBS for 2 x 5 min and incubated in the primary antibody at a dilution of 1: 3000 in TTBS for O/N at +4 °C with agitation. The antibody solution was decanted and the blot was rinsed briefly with TTBS and washed 4 x 5 min with TTBS at RT with agitation. Blot was incubated in secondary antibody (Amersham ECL Rabbit IgG, HRP-linked whole Ab, from GE Healthcare) diluted to 1:10 000 in for 2h at RT with agitation. The blot was washed 3 x 5 min with TTBS and 2 x 5 min with TBS and developed for 5 min with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare). Exposure time was around 2 min.

Courtesy of Tuomas Huokko, University of Turku, Finland


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