RPB1 | RNA polymerase II subunit B1

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AS11 1804 clonality: polyclonal host: rabbit reactivity:Arabidopsis thaliana



46 st
Item No:
AS11 1804

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product information

DNA-directed RNA polymerase II subunit 1 (EC= catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This enzyme is the central component of the basal RNA polymerase II transcription machinery. Synonymes:DNA-directed RNA polymerase III largest subunit,DNA-directed RNA polymerase II subunit RPB1.


KLH-conjugated synthetic peptide derived from known higher plant polymerase II sequences, including Arabidopsis thaliana P18616, At4g35800

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications immunoprecipitation (IP)
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Additional information

Purified IgG was lyophilized from PBS pH 7.4 + 0.02 % sodium azide. Therefore only distilled water is required for its re-constitution.

application information
Recommended dilution

1,2 or 5 µg  (IP)

Expected | apparent MW

204 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Amygdalus persica, Asparagus officinalis, Brassica rapa, Capsella rubella,  Glycine max, Hordeum vulgare, Solanum lycopersicum, Oryza glaberrima, Panicum italicum, Persea americana, Pisum sativum, Ricinus communis, Solanum tuberosum, Sorghum vulgare, Vitis vinifera
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references Dolata et al. (2015). NTR1 is required for transcription elongation checkpoints at alternative exons in Arabidopsis. EMBO J. 2015 Jan 7. pii: e201489478.

application example

IP using anti-plant polymerase II antibodies

Chromatin immunoprecipitation (ChIP). Chromatin immunoprecipitation was performed as described (Bowler et al. (2004), Plant Journal) with modifications. Sonic and IP buffer were prepared as described (Kaufmann et al. (2010)), Nature Protocols). Chromatin pellet was sonicated at 4 °C with a Diagenode Bioruptor set at high intensity for 10 min (30 sec on, 30 sec off intervals) to obtain 250-500 bp DNA fragments. Chromatin (20 µg) was immunoprecipitated with a RNA Pol II antibody (2µg/IP)and Dynabeads®Protein G (Life Technologies). Reverse corsslinking and elution of DNA fragments were performed using Chelex (Biorad) at 95°C, 10 min as described (Rowley et al. (2013), Methods). Sample without antibody was used to determine the nonspecific background pulled-down directly by the beads. Relative abundance of regions of interest in immunoprecipitated DNA was measured by real-time PCR. Red lines indicates amplified region. Values on the charts are shown as the mean ± SD level (% of input) of RNA Pol II on GAPDH gene (A) and MIR168a (B) gene. Data comes from three independent experiments.

Courtesy of MSc Jakub Dolata, Adam Mickiewicz University, Poland

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