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PPD2 | Protein PEAPOD 2

195 €

AS15 3107 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Item No:
AS15 3107

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product information
Background

PPD2 (protein PEABOD 2) belongs to the TIFY/JAZ family and is involved in lamina development, regulating leaf size and its curvature.

Alternative name: Protein TIFY4B

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana PPD2 sequence, Uniprot: Q8GY55 TAIR: At4G14720
Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

AS13 2648 | anti-JAZ1 | Jasmonate ZIM-domain protein 1 , rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Additional information

This antibody is recognizing recombinant PPD2 and reactivity on endogenous protein needs to be confirmed.

application information
Recommended dilution
Expected | apparent MW

34.8 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity
Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references

To be added when available, antibody released in September 2016.


Application example



Equal volumes of total proteins from E. coli purified with GST agarose beads or N. benthamiana were extracted and denatured with with Laemlli buffer (50 mM Tris pH 8, 10 mM EDTA, 18% glycerol, and 10 mM DTT) at 95°C for 10 min. Samples were separated on 12% SDS-PAGE and blotted 30 min to Protran nitrocellulose membrane (Whatman) using semi-dry transfer. Blots were blocked with 0.25% BSA/0.25% gelatin for 60 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 4 times for 15 min in 10mM Tris pH8.0, 0.3% Tween 20 buffer at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG alkaline phosphatase conjugated) diluted to 1:10000 in for 60 min at RT with agitation. The blot was washed as above and developed for 10 min with NBT/BCIP (nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate; Promega Corporation).

Courtesy of Dr. Garry Sunter, South Texas Center for Emerging Infectious Disease, San Antonio, USA

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