PPI | papain

194 €

AS09 545 | clonality: polyclonal | host: rabbit | reactivity: Carica papaya

product information

background

Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans. Alternative names: Papaya proteinase I, allergen= Car p 1.

immunogen

native papain isolated and purified from Carica papaya

antibody format

rabbit

polyclonal

purified IgG in phosphate buffer saline,

lyophilized

quantity

10 mg

for reconstitution please add 1 ml of sterile destilled water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)

related products

AS09 546 | anti-PPI | papain (biotinylated)

AS09 547 | anti-PPI | papain (affinity purified)

additional information

antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

application information
recommended dilution		1: 1000 - 1: 100 000 for techniques listed above
expected \| apparent MW		38.9 kDa
confirmed reactivity		Carica papaya
predicted reactivity		Carica papaya
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
selected references		to be added when available