ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

PR-1 | Positive control/quantitation standard

169 €
Buy 2 items of this product for 140 €/each
Buy 3 items of this product for 120 €/each

AS10 687S  |  Recombinant protein standard for quantification and positive control

When you buy this product you get a 50% discount on other protein standards.
Follow this link to a list of discounted standards.
To get this discount please send your order to orders@agrisera.com

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
19 st
Delivery:
Shipping:
Item No:
AS10 687S

Info: Product suggestions Add review
 
product information
Background

Pathogenesis-related protein 1 (PR-1) is partially responsible for acquired pathogen resistance. Induced by INA, salicylic acid and pathogen infection.

This product is a recombinant PR-1 protein, trunctated by first 26 amino acids, source: Arabidopsis thaliana,UniProt:P33154, TAIR: At2g14610

Host
Clonality
Clone
Purity
Format
Quantity 100 µl
Reconstitution
For reconstitution add 90 µl of milliQ water. Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications
Western Blot (WB)
Related products
AS10 687 | anti-PR-1 | Pathogenesis-related protein 1, rabbit antibody
Additional information

The PR-1 protein standard can be used in combination with anti-PR-1 antibodies to quantitate PR-1 protein. Quantitative western blot: detailed method descriptionvideo tutorial

application information
Recommended dilution
Standard curve: 3 loads are recommended eg.0.5, 2 and 4μl.
For most applications a sample load of 10-20 μg of protein will provide with a signal in this range.

Positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.

This standard is stabilized and ready and does not require heating before loading on the gel.

Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Expected | apparent MW

16.4 kDa

Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Concentration: after adding 90 µl of sterile milliQ water final concentration of the standard is 0.10 pmoles/µl

Protein standard buffer composition:
 Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.

This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.

This standard is stabilized and ready and does not require heating before loading on the gel.

Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Selected references

To be added when available


Application example

quantitative western blot using recombinant PR-1 protein

Recombinant PR-1 protein standard 0.05 pmol (1),  0.1 pmol (2), 0.15 pmol (3), 0.2 pmol (4), and 0.3 pmol (5) was loaded in each lane. Protein separation was done using NuPage 4-12% Tris-Bis gel (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2-2.5 % RPN2125 (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary anti-PR-1 antibody at a dilution of 1: 10 000 in blocking reagent for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602) diluted to 1:25 000 in TBS-T for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with TMA-6 (Lumigen) detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 1 minute.







||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com