PsaD | PSI-D subunit of photosystem I

320 €

AS09 461 | clonality: polyclonal | host: rabbit | reactivity: plants (monocots and dicots, conifers), moss: Physcomitrella patens, Chlamydomonas reinhardtii

PRODUCT INFORMATION IN PDF

product information

background

PsaD (PSI-D) is a core subunit of photosystem I highly conserved in all photosynthetic organisms (including bacteria with Fe-S type reaction centers). In eukaryots its encoded by 1 to 2 nuclear gene(s) and imported as a precursor into the chloroplast. In the thylakoid membrane it associates with PsaA and PsaB on the stromal site of the PSI core forming the Fd-docking site. PsaD is also required for the stable assembly of PsaC.

immunogen

KLH-conjugated synthetic peptide 100% conserved in all known plant PsaD sequences including Arabidopsis thaliana (At1g03130 and At4g02770) as well as Physcomitrella patens. The conservation in Chlamydomonas reinhardtii is high (14 of 16 aminoacids are identical).

antibody format

rabbit

polyclonal,

serum,

lyophilized

quantity

200 µl

- for reconstitution add 200 µl of sterile water

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products

PSI available antibodies to Photosystem I proteins

Photosynthesis available antibodies to photosynthetic proteins

additional information

PsaD has frequently been used as a marker for intact PSI reaction centers.

application information
recommended dilution		1 : 1000 with SuperSignal West Pico detection (WB)
expected \| apparent MW		17.9 \| 20 (for Arabidopsis thaliana)
confirmed reactivity		dicots: Arabidopsis thaliana, Spinacia oleracea, monocots: Hordeum vulgare, Oryza sativa, Zea mays, moss: Physcomitrella patens, algae: Chlamydomonas reinhardtii
predicted reactivity		plants (monocots, dicots and conifers), Bigelowiella natans, green algae
not reactive in		Synechococcus elongatus sp. PCC 7942
additional information		this antibody is a replacement for former product, anti-PsaD AS04 046
selected references		Heinnickel et al. (2013). Novel thylakoid membrane greencut protein cpld38 impacts accumulation of the cytochrome b6f complex and associated regulatory processes. J. Biol. Chem. Jan 9. Yadavalli et al. (2012). Differential degradation of photosystem I subunits under iron deficiency in rice. J Plant Physiol. March 22. Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source)

application example

10 µg of total leaf protein extracted with PEB (AS08 300) from (1) Zea mays, (2) Chlamydomonas reinhardtii, and (3) Spinacia oleracea were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 80 min (30V) to nitrocellulose. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PsaD (AS09 461, 1:1000, 1h) and secondary anti-rabbit (1:40000, 1h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with SuperSignal West Pico (Thermo Scientific) using a GenoPlex Chemi CCD (accumulated signal 10 x 30s exposure, bin 2x2).

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