Plant/Algal cell antibodies / Photosynthesis / PSII (Photosystem II)

product information

The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1

KLH-conjugated synthetic peptide, amino acids 234-242 of D1 protein P83755

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS05 084 | PsbA antibody, C-terminal rabbit antibody

AS05 084-10 | PsbA antibody, smaller unit size of AS05 084

AS01 016 | anti-PsbA C-terminal hen antibody

AS06 124A | anti-PsbA, N-terminal rabbit antibody

AS01 016S | PsbA protein standard for quantitation and positive control

antibody will detect 23 kDa N-terminal fragment

application information

1 : 10 000  on thylakoid fraction with standard ECL(WB)

38 | 28-30 kDa 

dicots: A.thaliana, M. truncatula, S. vulgaris, S. oleracea, monocots: H. vulgare,  moss: P. patens, algae: Ch. reinhardii, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803

dicots including:  Glycine max, Nicotiana tabacum, Pisum sativum, Ricinus communis, Vitis vinifera, monocots including: Oryza sativa, Zea mays, trees: Populus balsamifera

no confirmed exceptions from predicted reactivity known in the moment

antibody is recognizing a 23 kDa fragment in spinach and Arabidopsis thylakoids

for usage on total cell extracts the dilution needs to be determined experimentally

Yoshioka et al. (2010).  Quality control of photosystem II: FtsH hexamers are localized near photosystem II at grana for the swift repair of damage. J Biol Chem 285(53): 41972-41981.

Lundin et al. (2007). The Arabidopsis PsbO2 protein regulates dephosphorylation and turnover of the photosystem II reaction centre D1 protein. Plant J. 49: 528-539

Spetea et al. (1999).  GTP bound to chloroplast thylakoid membranes is required for light-induced, multienzyme degradation of the photosystem II D1 protein. PNAS 96: 6547-6552.

Thylakoid membranes from Arabidopsis (0.05-0.75 µg of Chl) were separated on 14%AA+ 6M urea gels and blotted 1h to PVDF. Blots were blocked immediately following transfer in 5% milk solution for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 20 000 o.n. at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (donkey anti-rabbit IgG horse radish peroxidase conjugated, from GE-Healthcare) diluted to 1:5 000 . The blots were washed as above and developed for 5 min with ECL-Plus detection reagent according to the manufacturers instructions. Exposure time was 1 min in CCD camera Fuji4000.

Courtesy Professor Cornelia Spetea-Wiklund