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PsbB | CP47 protein of PSII

350 €

AS04 038 | clonality: polyclonal | host: rabbit | reactivity: [global antibody] for higher plants, Physcomitrella patens, algae, cyanobacteria, diatoms

PRODUCT INFORMATION IN PDF

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Item No:
AS04 038

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product information
Background

PsbB (CP47) is a chlorophyll-binding protein located in the membrane, where it serves as the core antenna of Photosystem II.

Immunogen

KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbB sequences including Arabidopsis thaliana AtCg00680, Hordeum vulgare P10900, Oryza sativa P0C364, Synechocystis PCC 6803 P05429

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

AS04 038S | PsbB protein standard for quantitation and positive control is discontinued. We recommend to use PsbD | D2 together with PsbD antibody

AS04 038PRE | PsbB | CP47 protein of PSII, pre-immune serum
antibodies to other PSII proteins

recommended secondary antibody

Plant and algal protein extraction buffer

Secondary antibodies

Additional information This antibody can be used as a loading control for studies of PSIi or photosynthetic acclimation in diatoms Blommaert et al. 2017.  Limnol. Oceanogr. DOI: 10.1002/lno.10511.
application information
Recommended dilution

1: 2000 (WB)

Expected | apparent MW

56 kDa

Confirmed reactivity

Anabaena 7120, Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Malus prunifolia, Opephora
guenter-grassii
(diatom), Oryza sativa, Phaseolus vulgaris, Physcomitrella patens, Pisum sativum, Synechococcus PCC7942, 6803, , Seminavis robusta (diatom)

Predicted reactivity

Abies concolor, Brachypodum distachyon, Brassica napus, cyanobacteria, Cucumis sativus, Ephedra sp., Glycine max, Lotus japonicus, Nanochloropsis sp. , Nicotiana tabacum, Panax ginseng, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor,  Spinacia oleracea, Triticum aestivum, Vitis vinifera

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This product can be sold containing ProClin if requested

in bis-tris gel systems PsbB protein migrates between 40-45 kDa

Selected references Blommaert et al. (2017). Contrasting NPQ dynamics and xanthophyll cycling in a motile and a non-motile intertidal benthic diatom. Limnol. Oceanogr. doi: 10.1002/lno.10511
Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.
Hu et al. (2017). The SUFBC2 D Complex is Required for the Biogenesis of All Major Classes of Plastid Fe-S Proteins. Plant J. 2017 Jan 19. doi: 10.1111/tpj.13483.
Fan et al. (2016). Proteome Analyses Using iTRAQ Labeling Reveal Critical Mechanisms in Alternate Bearing Malus prunifolia. J Proteome Res. 2016 Oct 7;15(10):3602-3616.
Kowalewska et al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015.
Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113
Liu and Last (2015). A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in Arabidopsis thaliana. Plant J. 2015 Jun;82(5):731-43. doi: 10.1111/tpj.12845. Epub 2015 Apr 29.
Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.
Bhuiyan
 et al. (2015). MET1 Is a Thylakoid-Associated TPR Protein Involved in Photosystem II Supercomplex Formation and Repair in Arabidopsis. Plant Cell. 2015 Jan 13. pii: tpc.114.132787.
Sun et al. (2014). Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes. Biochim Biophys Acta. 2014 Nov 22. pii: S0005-2728(14)00650-1. doi: 10.1016/j.bbabio.2014.11.005.

application example

2 µg of total protein from (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300), (2) Horderum vulgare leaf extracted with PEB (AS08 300), (3) Chlamydomonas reinhardtii total cell extracted with PEB (AS08 300), (4) Synechococcus sp. 7942 total cell extracted with PEB (AS08 300), (5) Anabaena sp. total cell extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

 

Western blot of anti-PsbB antibody

application example

0.2 µg of chlorophyll from (4,5) Arabidopsis thaliana leaf extracted with PEB (AS08 300), (1) 500 fmol of PsbB protein standard, (2) 200 fmol of PsbB protein standard, (3) 75 fmol of PsbB protein standard were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

 

western blot detection using anti-PsbB antibody




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