LEA4-25 | Group 4-late embryogenesis abundant protein

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AS13 2757 clonality: polyclonal host: rabbit reactivity: Phaseolus vulgaris


25 st
Item No:
AS13 2757

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product information
Background LEA4-25 (Group 4-late embryogenesis abundant protein PvLEA4-25) belongs to a group of very hydrophilic proteins, described over 25 years ago as accumulating during late stages of plant seed development. Found in vegetative plant tissues following exposure to environmental stress. Synonymes: Putative late embryogenesis abundant protein LEA.

Recombinant Phaseolus vulgaris PvLEA 4-1 sequence derived from Pv01G142000

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS13 2758 | anti-LEA4-5 | Late embryogenesis abundant protein 4-5, rabbit antibodies
AS13 2756 | anti-LEA6-3 | late embryogenesis abundant protein 6-3, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Additional information

This antibody does not recognize group 4 LEA proteins from Arabidopsis thaliana.

application information
Recommended dilution

1 : 2000 with standard ECL (WB)

Expected | apparent MW

16 | 18 kDa

Confirmed reactivity

Phaseolus vulgaris

Predicted reactivity Glycine tomentosa, Glycine max
Not reactive in

Arabidopsis thaliana

Additional information The PvLEA4-1 protein from common bean presents a deduced molecular mass of 16 kDa; however, as other LEA and disordered proteins it migrates with an apparent higher molecular mass, possibly due to post-translational modifications.
Selected references

to be added when available, antibody released in November 2014.

application information

western blot using anti-LEA4-25 antibodies

Phaseolus vulgaris seed protein extracts which is  an extract enriched in hyrdophilic proteins, obtained in a following way: Dry seeds were used to separate embryos from cotyledons. Approximately 500 mg of embryos were ground in a mortar in the presence of liquid nitrogen or dry ice. The powder was resuspended and homogenized in 1 mL of 20 mM TES pH 8.0, 0.5 M NaCl, 10 mM Na2VO3, 10 mM NaF, 1 mM PMSF and one tablet/10 mL (protease inhibitor cocktail tablet, Roche). The suspension was centrifuged at 14,000 rpm in a microcentrifuge during 10 min at 4ºC, supernatant was separated and centrifugation was repeated once more. Then, supernatant was boiled 10 min and transfer to ice for 15 min, the suspension was centrifuged at 14,000 rpm in a microcentrifuge during 10 min at 4ºC, and the boil-ice-centrifugation procedure was repeated twice. Subsequently, the supernatant was dialyzed against 10 mM phosphate buffer pH 8.0 in the cold room. Concentration of the protein extract was determined by standard procedures and its integrity was verified by SDS-PAGE. 
Following samples were separated: PvLEA4 is a recombinant PvLEA4-1 proein which does not contain any tag and was purified from bacterial extract. M: monomoer, D: dimer. DE*: total protein extract from dry Phaseolus vulgaris embryos. Proteins were separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry transfer for 1 h. Blots were blocked ON at 4ºC in 2% non-fat milk with agitation. Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 2% non-fat milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 min.

Courtesy of Dr. Alejandra A. Covarrubias, UNAM, Mexico

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