PYR1 | Abscisic acid receptor RCAR11

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AS13 2634 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


27 st
Item No:
AS13 2634

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product information

PYR1 (Abscisic acid receptor RCAR11) is a member of PYR (pyrabactin resistance) family, which function as abscisic acid sensors. Alternative names: ABI1-binding protein 6, Protein PYRABACTIN RESISTANCE 1, Regulatory components of ABA receptor 11.


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PYR1 sequence, UniProt: O49686, TAIR: At4g17870

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS12 1861 | anti-ABI1 | abscisic acid insensitive 1, rabbit antibodies
AS12 1871 | anti-ABI2 | abscisic acid insensitive 2, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Additional information

to be added when available

application information
Recommended dilution

1 : 10 000 with standard ECL (WB)

Expected | apparent MW

21 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Brassica sp.
Not reactive in no confirmed exceptions from predicted reactivity are currently known
Additional information

to be added when available

Selected references

to be added when available, antibody released in November 2014.

application example

western blot using anti-PYR1 antibodies

150 µg of total protein from Arabidopsis thaliana (col-0)  extracted with  50mM Tris, 150mM NaCl, 05%Triton X-100, 2mM DTT, 1mM PMSF, protease inhibitor were separated on  10 % SDS-PAGE  and blotted 2h to PVDF using semi-dry. Blots were blocked with 1xTBST with nonfat milk 5% for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:50 000 in  for 1h at RT with agitation. The blot was washed for 15 min  and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 5 min. Longer exposure time for wt samples is necessary.

Courtesy, Dr. Joanna Kufel, Institute of Genetics and Biotechnology, Warsaw University

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