Rabbit anti-Bovine IgG (H&L), DyLightŪ 633 conjugated

190 €
AS16 3250 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Bovine IgG (H&L)


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Item No:
AS16 3250

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product information

Rabbit anti-Bovine IgG (H&L), DyLight® 633 conjugated is a secondary antibody conjugated to  DyLight® 633, which binds to Bovine IgG (H&L) in immunological assays. 
DyLight® 633 has Amax = 638 nm, Emax = 658 nm.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Immunogen Purified Bovine IgG, whole molecule
Host Rabbit
Clonality Polyclonal
Purity Affinity purified
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water.
Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store freeze-dried powder at 2-8 °C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications Flow cytometry (FACS), Immunomicroscopy (IM)
Related products

other anti-bovine, dylight conjugated, secondary antibodies

Additional information Antibody purity is > 95% based on SDS-PAGE,
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on IEP, this antibody reacts with: · heavy (γ) chains on bovine IgG · light chains on all bovine immunoglobulins.
Based on IEP, no reactivity is observed to: · non-immunoglobulin bovine serum immunoglobulins.
application information
Recommended dilution 1:20 - 1:2 000 (FACS, IM), optimal working dilution should be determined by the investigator.
Expected | apparent MW
Confirmed reactivity

Bovine IgG

Predicted reactivity
Not reactive in
Additional information
Selected references To be added when available, antibody released in April 2016.

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