Rabbit anti-Chicken IgY (H&L), ALP conjugated

197 €
AS10 1486 | clonality: polyclonal | host: rabbit | reactivity: chicken IgG/Y (H&L)


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Item No:
AS10 1486

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product information
Background Rabbit anti-chicken IgG/Y (H&L) is a secondary antibody conjugated to ALP, which binds to chicken IgG/Y (H&L) in immunological assays. Antibody is affinity purified using solid phase chicken IgG/Y.
Immunogen Purified Chicken IgG/Y (H&L)
Host Rabbit
Clonality Polyclonal
Purity Affinity purified Rabbit IgG
Format Liquid
Quantity 1 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Related products other secondary antibodies against chicken IgG/Y (H&L)
Tested applications
Additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.

Antibody has been affinity purified using solid phase chicken IgG/Y.

Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin chicken serum proteins.

application information
Recommended dilution This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:2000 for immunohistochemistry 1:50 000-1:5 000 for ELISA/Western blot.
Selected references

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