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Rabbit anti-Chicken IgY (H&L), ALP conjugated

197 €
AS10 1486 | clonality: polyclonal | host: rabbit | reactivity: chicken IgG/Y (H&L)

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AS10 1486

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product information
Background Rabbit anti-chicken IgG/Y (H&L) is a secondary antibody conjugated to ALP, which binds to chicken IgG/Y (H&L) in immunological assays. Antibody is affinity purified using solid phase chicken IgG/Y.
Immunogen Purified Chicken IgG/Y (H&L)
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified Rabbit IgG
Format Liquid
Quantity 1 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products other secondary antibodies against chicken IgG/Y (H&L)
Additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.

Antibody has been affinity purified using solid phase chicken IgG/Y.

Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin chicken serum proteins.

application information
Recommended dilution This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:2000 for immunohistochemistry 1:50 000-1:5 000 for ELISA/Western blot.
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
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