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Rabbit anti-Chicken IgY (H&L), HRP conjugated- free sample

AS10 1489-free sample | clonality: polyclonal | host: rabbit | reactivity: chicken IgG/Y (H&L)

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AS10 1489-free

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product information
Background Rabbit anti-chicken IgG/Y (H&L) is a secondary antibody conjugated to HRP, which binds to in immunological assays. Antibody is affinity purified using solid phase chicken IgG/Y.
Immunogen Purified Chicken IgG/Y (H&L)
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified Rabbit IgG
Format
Quantity 10 µl (0.91 mg/ml)
Reconstitution
Storage Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Rehydrate with 1.1 ml of deionized water. Prepare fresh working dilution daily.
Tested applications
Related products other secondary antibodies against chicken IgG/Y (H&L)
Additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.

Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin chicken serum proteins

application information
Recommended dilution This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:5 000 for Immunohistochemistry 1:200-1:5000 for ELISA/Western blot
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references


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