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Rabbit anti-Goat IgG Fc, ALP conjugated

203 €

AS10 1254   | clonality: polyclonal  |  host:rabbit   |  reactivity:goat IgG Fc

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AS10 1254

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product information
Background Rabbit anti-goat IgG Fc, ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to IgG Fc constant part in immunological assays. Antibodies are affinity purified using solid phase goat IgG.
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified IgG
Format Liquid
Quantity 0.5 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products other ALP conjugated secondary antibodies to goat IgG
Additional information

This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis.

Minimum cross-reactivity is observed to the light chains or to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

application information
Recommended dilution 1:500-1:2000 for ELISA/western blot, 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC).
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references


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