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Rabbit anti-Goat IgG (H&L), ALP conjugated

216 €

AS09 630 | clonality: polyclonal  |  host: rabbit  | reactivity: goat IgG (H&L)

PRODUCT INFORMATION IN PDF

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AS09 630

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product information
Background

Rabbit anti-goat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all goat immunoglobulins in immunological assays.

Immunogen

Purified Goat IgG, whole molecule

Host Rabbit
Clonality Polyclonal
Clone
Purity Purified rabbit IgG
Format Liquid
Quantity 1 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC)
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Additional information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

application information
Recommended dilution

1: 500 - 2 000 (ELISA) and  (WB), 1: 20  -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)

Expected | apparent MW
Confirmed reactivity

goat IgG heavy and light chains (H&L)

Predicted reactivity

goat IgG heavy and light chains (H&L)

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

No reactivity is observed to non-immunoglobulin goat serum proteins based in immunoelectrophoresis.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Selected references

to be added when available



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