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Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, ALP conjugated, min. cross-reactivity to human, mouse,Rat IgG

209 €

AS10 1064  | clonality: polyclonal  |  host: rabbit  |  reactivity:goat IgG (H&L)

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AS10 1064

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product information
Background Rabbit anti-goat IgG (H&L) is a F(ab)'2 fragment of ALP (alkaline phosphatase) conjugated secondary antibody which binds to all goat IgGs in immunological assays. Antibody is adsorbed to human,mouse,rat IgG and has purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Immunogen
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified IgG, F(ab)'2 fragment
Format Liquid
Quantity 0.5 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Related products other ALP conjugated secondary antibodies to goat IgG
Tested applications
Additional information

This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse or rat based on immunoelectrophoresis.

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

application information
Recommended dilution 1:500-1:2000 for ELISA/western blot, 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC).
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