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Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, FITC conjugated, min. cross-reactivity to human, mouse,Rat IgG

216 €

AS10 1058 | clonality: polyclonal  |  host: rabbit  |  reactivity:goat IgG (H&L)

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AS10 1058

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product information
Background

Rabbit anti-goat IgG (H&L) is a secondary antibody F(ab)'2 fragment, which binds to all goat IgGs in immunological assays and is FITC (fluorescein-5-isothiocyanate) conjugated. Antibody is also specially adsorbed against human,mouse and rat IgG.  FITC  Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is > 90% based on SDS-PAGE, may contain small amounts of intact IgG.

Immunogen
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified IgG, F(ab)'2 fragment
Format Liquid
Quantity 0.5 mg
Reconstitution
Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Related products other fluorescent antibodies to goat IgG
Tested applications
Additional information

This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse or rat based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

application information
Recommended dilution

1:20-1: 2000 for immunohistochemistry (IHC)

1:50-1: 5000 for immunocytochemistry (ICC)

Selected references


||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at support@agrisera.com