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Rabbit anti-Mouse IgG (H&L), HRP conjugated

181 €

AS09 627 | clonality: polyclonal  |  host: rabbit  | reactivity: mouse IgG (H&L)

PRODUCT INFORMATION IN PDF

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Item No:
AS09 627

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product information
Background

Rabbit anti-mouse IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins in immunological assays.

Immunogen

Purified Mouse IgG, whole molecule

Host Rabbit
Clonality Polyclonal
Clone
Purity Purified rabbit IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
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Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative.

application information
Recommended dilution 1: 10 000 -1: 50 000 (ELISA), 1: 500 -1: 5000 (IHC), 1: 5 000 (WB)
Expected | apparent MW
Confirmed reactivity mouse IgG heavy and light chains (H&L)
Predicted reactivity

mouse IgG heavy and light chains (H&L)

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis

Selected references

to be added when available



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