Plant/Algal cell antibodies / Photosynthesis / RUBISCO

product information

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.

KLH-conjugated synthetic peptide derived from all known plant,algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, including Arabidopsis thaliana (AtCg00490), Sychococcus sp. Q3ALL1

store at 4°C; make aliquots to avoid working with a stock. Please, Remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

western blot (WB) immunolocalization (IL)

AS01 017S | Rubisco protein standard for quantitation

AS03 037 | Anti-RbcL rabbit antibody

secondary antibody to use with this product:

Goat anti-chicken IgY (H&L), HRP conjugated

or

Goat anti-chicken IgY (H&L), ALP conjugated

peptide target used to elicit this antibody is not conserved in type II Rubisco found in dinoflagellates and some photosynthetic bacteria. For those species product AS03 037 is recommended.

application information

1: 10 000 - 1: 20 000 on 2 µg of total cellular protein, detected with standard ECL (WB); (IL) tested on a grass species, formaldehyde-fixed and paraffin-embedded tissue  following the protocol from Gonzalez et al. (1998) Plant Physiol. V. 116

52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)

Arabidopsis thaliana, Lobaria pulmonaria, Medicago sativa, mixed phytoplankton, Pisum sativum, Solanum tuberosum, Spartina alterniflora, Spinacia oleracea, Synechococcus sp. PCC7842, Thiobacillus sp. Ulmus sp.

di and monocots, green algae, mosses, conifers, liverworts, welwitschia, prochlorophytes

no confirmed exceptions from predicted reactivity known in the moment

This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).

Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.

MacKenzie et al. (2005) Inorganic carbon acclimation in Synechococcus elongatus alters the dynamics of macromolecular pooks and photosynthetic fluxes in response to increased light. Photosynt Research 85: 341-357.

Schofield et al. (2003) Changes in macromolecular allocation in nondividins algal symbionts allow for photosynthetic acclimation in the lichen Lobaria pulmonaria. New Phytol 159: 709-718