RBR1 | Retinoblastoma related protein
AS11 1627 | clonality: polyclonal | host: chicken | reactivity: Arabiodpis thaliana, Medicago sativa
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1 : 6000 with standard ECL (WB), 2µl (IP)
|Expected | apparent MW||
Arabidopsis thaliana, Medicago sativa
|Predicted reactivity||dicots: Camelia sinensis, Chenopodium rubrum, Pisum sativum, Scutelaria baicalensis, monocots: Cocos nucifera, Hordeum vulgare, Zea mays, Oryza sativa, trees: Populus tremula|
|Not reactive in||
Antibody is not suitable for immunolocalization.
|Selected references||Cheng et al. (2013). Down-regulation of multiple CDK inhibitor ICK/KRP genes up-regulates E2F pathway and increases cell proliferation, organ and seed sizes in Arabidopsis. Plant j. May 7.
Ábrahám et al. (2011). Immunodetection of retinoblastoma-related protein and its phosphorylated form in interphase and mitotic alfalfa cells. J Exp Bot 62(6):2155-2168.
35 µg of total proteins from Arabidopsis thaliana Col-0 cell suspension culture (1) or one week old seedlings (2) as well as 3.5ng of purified GST-RBR1 fusion protein (3) were separated on 8% Laemmli SDS polyacrylamide gels and blotted onto PVDF membrane overnight. Filters were blocked in 5% milk powder in TBS-0.05% Tween 20 (TBS-T) for 2 hours then probed with anti-RBR1 antibody (1:6000, 2 hours at RT) and HRP-conjugated rabbit anti-chicken IgY (PIERCE) secondary antibody (1: 20000, 1 hour at RT) in TBS-T containing 5% milk powder. After each antibody incubation steps filters were washed with TBS-T, TBS-T containing 2% milk powder, TBS-T for 10 min each on a rocking platform. Signal was developed with Super Signal West Pico chemiluminescent substrate (PIERCE) and visualized by exposing to a film (Agfa Cronex 5) for 5 min.
Courtesy Dr. Laszlo Bako, Umeå Plant Science Center, Sweden
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