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RCD1 | Radical cell death 1

265 €
Buy 2 items of this product for 198 €/each
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AS12 2602 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

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AS12 2602

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product information
Background RCD1 (Radical cell death 1) functions as a regulator of oxidative-stress hormonal and developmental responses. Alternative name: Inactive poly [ADP-ribose] polymerase RCD1.
Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RCD1 protein sequence UniProt: Q8RY59, TAIR: AT1G32230

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 100 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB) following (IP)
Related products

collection of antibodies to proteins involved in oxidative stress 

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with ECL (WB) on samples following IP

Expected | apparent MW

65 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity please inquire
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

Protein has three splice variants: 63.8 + 65.6 + 65.7 kD

Peptide used to elicit this antibody was designed for detection of all (3) RCD1 splice variants but NOT the close relative SRO1.

Selected references

to be added when available, antibody released in October 2015. 


 application information 

western blot using anti-RCD1 antibodies

5 mg of total protein from Arabidopsis thaliana seedlings expressing RCD1-HA under native promoter extracted with RIPA buffer for protein extraction (50 mM Tris-HCl pH 7,5, 150 mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate, protease inhibitor cocktail 1:100, 50 uM MG132) were used for immunoprecipitation and afterwards separated on 10% SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the anti-HA antibody (Roche) at a dilution of 1: 1 000 in 1% milk, TBS-T overnight at +4C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rat IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 1% milk TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL Prime, GE Healthcare. Exposure time was 1 min.

Courtesy Dr. Julia Vainonen, Helsinki University, Finland

immunoprecipitation using anti-RCD1 antibodies
5 µl of anti-RCD1 antibody was used for immunoprecipiation. Samples: transgenic line expressing HA-tagged RCD1 (Jaspers et al., 2009, Plant J) (1), rcd1-4 mutant (2). Afterwards these samples were separated on 10% SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the anti-HA antibody (Roche) at a dilution of 1: 1 000 in 1% milk, TBS-T overnight at +4C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rat IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 1% milk TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL Prime, GE Healthcare. Exposure time was 1 min.

Courtesy Dr. Julia Vainonen, Helsinki University, Finland

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com