RFA | Baker's yeast replication factor A
AS07 214 | clonality: polyclonal | host: rabbit | reactivity: Saccharomyces cerevisiae
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1: 20 000 with standard ECL (WB), chromatin immunoprecipiation
|Expected | apparent MW||
70 + 30 + 14 kDa
|Confirmed reactivity||Saccharomyces cerevisiae|
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
Antibody was also successfully used in ChIP application; presented data are courtesy of M. Pool and Dr. H. van Attikum.
|Selected references||Yeeles et al. (2015). Regulated eukaryotic DNA replication origin firing with purified proteins. Nature. 2015 Mar 4. doi: 10.1038/nature14285.
Holstein et al. (2014). nterplay between Nonsense-Mediated mRNA Decay and DNA Damage Response Pathways Reveals that Stn1 and Ten1 Are the Key CST Telomere-Cap Components. Cell Rep. 2014 May 22;7(4):1259-69. doi: 10.1016/j.celrep.2014.04.017. Epub 2014 May 15.
Deng et al. (2014). RPA antagonizes microhomology-mediated repair of DNA double-strand breaks. Nat Struct Mol Biol. 2014 Mar 9. doi: 10.1038/nsmb.2786.
TCA precipitated protein extracts from a wild type yeast strain (S. cerevisiae) were separated on 10% gel and transferred to a PVDF membrane. Antibody was used in different dilutions: 1: 5000 (1); 1: 10 000 (2); 1: 20 000 (3);
Besides the bands for RFA1 and RFA2 an unspecific band was detected at ~150 kDa.
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