ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

RGL2 | RGA-like protein 2 (DELLA protein)

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS11 1803 | clonality: polyclonal | host: rabbit | reactivity:Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
15 st
Delivery:
Shipping:
Item No:
AS11 1803

Info: More information Product suggestions Add review
product information
Background

DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal, stamen and anther development. Predominantly expressed in germinating seeds, flowers and siliques.  Synonymes: GRAS family protein 15, AtGRAS-15, Scarecrow-like protein 19, AtSCL19.

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RGL2 protein, UniProt: Q8GXW1, TAIR: At3g03450

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS13 2638 | anti-GID2 | F-BOX protein GID2 (SLEEPY 1), rabbit antibody
AS12 1860 | anti-PIF4 | Phytochrome interacting factor 4, rabbit antibody

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with standard ECL (WB) on transfected Arabidopsis thaliana protoplasts

Expected | apparent MW

60 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity
Not reactive in

Triticum sp.

Additional information

As this protein is expressed in tissue in very low levels, please load at least 50 µg/well.
Recommended extraction and denaturation protocol: 

Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95°C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade. 
Selected references

to be added when available, antibody released in June 2014.



||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com