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RIQ1 | Reduced induction of non-photochemical quenching 1

265 €
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AS15 2835  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

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AS15 2835

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product information
Background RIQ1 is involved in regulation of thylakoid structure. 
Immunogen KLH-conjugated peptide, derived from Arabidopsis thaliana RIQ1, UniProt: Q8VYV1, TAIR: AT5G08050
Host Rabbit
Clonality Polyclonal
Clone
Purity

Affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Quantity

50 µg

Reconstitution

For reconstitution add 50 ĩl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

western blot (WB)

Related products
Additional information
application information
Recommended dilution

1:1000 (WB)

Expected | apparent MW

16 | 16 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

higher plants

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references To be added when available, antibody released in October 2016.

Application example


western blot using anti-RIQ1 antibodies


10 μg of total protein from Arabidopsis thaliana young seedlings total plant extract were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane using tank transfer. Blots were blocked with 10% Milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated, AS09 602 from Agrisera ) diluted to 1:10 000 in TTBS for 1h at RT. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 minute.

Courtesy of Dr. Rikard Fristedt VU University Amsterdam, The Netherlands

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