Rpl1 | 50S ribosomal protein L1

345 €

AS11 1738   | clonality: polyclonal  |  host: rabbit  |  reactivity:cyanobacteria


9 st
Item No:
AS11 1738

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product information

Rpl1 (50S ribosomal protein L1) belongs to the ribosomal protein L1P family. This protein binds directly to 23S rRNA, acts also as a translational repressor protein. 


KLH-conjugated synthetic peptide derived from all known cyanobacterial Rpl1 sequences including Synechocystis sp. 6803, P36236

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 2000 with standard ECL (WB)

Expected | apparent MW

25 kDa

Confirmed reactivity

Anabaena sp., Synechococcus sp. 7942, Synechocystis sp. 6803

Predicted reactivity all known cyanobacterial species
Not reactive in


Additional information

to be added when available

Selected references

Linhartová et al. (2014).  Accumulation of the Type IV prepilin triggers degradation of SecY and YidC and inhibits synthesis of Photosystem II proteins in the cyanobacterium Synechocystis PCC 6803. Mol Microbiol. 2014 Jul 24. doi: 10.1111/mmi.12730.

application example

western blot detection using cyanobacterial anti-Rpl1 antibody

5 µg of total protein from Anabaena sp. (1), Synechococcus sp. 7942 (2), Synechocystis sp. 6803 (3) extracted with Agrisera protein extraction buffer PEB  were separated on 4-12% NuPAGE and blotted 1h to PVDF. Blots were blocked with ECL Advance Blocking Reagent for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:50 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL Advance according to the manufacturers instructions (GE Healthcare). Exposure time was 180 seconds.

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