RPL1 | 50S ribosomal protein L1
AS11 1738 | clonality: polyclonal | host: rabbit | reactivity:cyanobacteria
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1 : 2000 with standard ECL (WB)
|Expected | apparent MW||
Anabaena sp., Synechococcus sp. 7942, Synechocystis sp. 6803
|Predicted reactivity||all known cyanobacterial species|
|Not reactive in||
to be added when available
Linhartová et al. (2014). Accumulation of the Type IV prepilin triggers degradation of SecY and YidC and inhibits synthesis of Photosystem II proteins in the cyanobacterium Synechocystis PCC 6803. Mol Microbiol. 2014 Jul 24. doi: 10.1111/mmi.12730.
5 µg of total protein from Anabaena sp. (1), Synechococcus sp. 7942 (2), Synechocystis sp. 6803 (3) extracted with Agrisera protein extraction buffer PEB were separated on 4-12% NuPAGE and blotted 1h to PVDF. Blots were blocked with ECL Advance Blocking Reagent for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:50 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL Advance according to the manufacturers instructions (GE Healthcare). Exposure time was 180 seconds.
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