RPL2 | ribosomal protein L2 (chloroplastic)

350 €

AS15 2876  | clonality: polyclonal  |  host: rabbit  |  reactivity: A.thaliana, H. vulgare, N. Tabacum. S. lycopersicum, S. oleracea


17 st
Item No:
AS15 2876

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product information
Background Ribosomal protein L2, chloroplastic, is one of the primary proteins involved in rRNA-binding and is a constituent of the large subunit of the ribosomal complex.

Full length, recombinant RPL2 protein from Arabidopsis thaliana, Uniprot: P56791, TAIR:ATCG00830

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl

For reconstitution add 100 µl of sterile water.

Lyophilized antibody can be stored at -20 or -80°C.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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Secondary antibodies

Additional information
application information
Recommended dilution

1:3000 (WB)

Expected | apparent MW

29.9 kDa

Confirmed reactivity

Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Spinacia oleracea

Predicted reactivity Brassica napus, Capsella rubella, Citrus sinensis, Cucumis sativus, Gossypium arboreum, Populus trichocarpa, Solanum lycopersicum, Solanum tuberosum, Ricinus communis, Vitis vinifera
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information The RPL2 protein is a small protein that should be best analysed using 14% SDS PAGE as described by Schägger (2006, Nature Protocols, 1, 16-20).
Selected references to be added when available, antibody released in May 2015

application example

western blot using anti-plant RPL2 antibodies

20 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 5 seconds.

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