RPL2 | Ribosomal protein L2 (chloroplastic)
AS15 2876 | clonality: polyclonal | host: rabbit | reactivity: A.thaliana, H. vulgare, N. Tabacum. S. lycopersicum, S. oleracea
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|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Spinacia oleracea|
|Predicted reactivity|| Brassica napus, Capsella rubella, Citrus sinensis, Cucumis sativus, Gossypium arboreum, Populus trichocarpa, Solanum lycopersicum, Solanum tuberosum, Ricinus communis, Vitis vinifera
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
|Additional information||The RPL2 protein is a small protein that should be best analysed using 14% SDS PAGE as described by Schägger (2006, Nature Protocols, 1, 16-20).
Following sucrose gradient centrifugation of maize chloroplasts this antibody detects two bands that are very similar in size.
|Selected references||to be added when available, antibody released in May 2015|
20 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 5 seconds.
Note: Solanum lycopersicum requires higher load/well and another western blot protocol. If you intend to use this antibody on this species, you are welcome to contact us.
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