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RPL2 | Ribosomal protein L2 (chloroplastic)

361 €

AS15 2876  | clonality: polyclonal  |  host: rabbit  |  reactivity: A.thaliana, H. vulgare, N. Tabacum. S. lycopersicum, S. oleracea

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AS15 2876

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product information
Background Ribosomal protein L2, chloroplastic, is one of the primary proteins involved in rRNA-binding and is a constituent of the large subunit of the ribosomal complex.
Immunogen

Full length, recombinant RPL2 protein from Arabidopsis thaliana, Uniprot: P56791, TAIR:ATCG00830

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution
Storage

For reconstitution add 100 µl of sterile water.

Lyophilized antibody can be stored at -20 or -80°C.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
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Secondary antibodies

Additional information
application information
Recommended dilution

1:3000 (WB)

Expected | apparent MW

29.9 kDa

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Spinacia oleracea
Predicted reactivity Brassica napus, Capsella rubella, Citrus sinensis, Cucumis sativus, Gossypium arboreum, Populus trichocarpa, Solanum lycopersicum, Solanum tuberosum, Ricinus communis, Vitis vinifera
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information The RPL2 protein is a small protein that should be best analysed using 14% SDS PAGE as described by Schägger (2006, Nature Protocols, 1, 16-20).

Following sucrose gradient centrifugation of maize chloroplasts this antibody detects two bands that are very similar in size.
Selected references to be added when available, antibody released in May 2015

application example

western blot using anti-plant RPL2 antibodies

20 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 5 seconds.

Note: Solanum lycopersicum requires higher load/well and another western blot protocol. If you intend to use this antibody on this species, you are welcome to contact us.

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com