RpoC1 | RNA polymerase beta' subunit (chloroplast)

Name: RpoC1 | RNA polymerase beta' subunit (chloroplast)
SKU: AS15 2868
Price: 319 EUR
Availability: InStock
Rating: 5 (2 reviews)

Product no: AS15 2868

AS15 2868 | Clonality: Polyclonal | Host: Rabbit | Reactiviy: Arabidopsis thaliana, Zea mays

319 €

DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Immunogen:	His-tagged, highly conserved fragment of Zea mays RpoC.
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	100 �g
Reconstitution:	For reconstitution, add 50 �l of sterile water
Storage:	Store lyophilized/reconstituted at -20�C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 500-1 : 1000 (WB)
Expected \| apparent MW:	78 \| 78 kDa

Reactivity

Confirmed reactivity:	Arabidopsis thaliana, Zea mays
Predicted reactivity:	Alloteropsis semialata, Aristida purpurea, Chasmanthium latifolium, Chaetobromus involucratus subsp. dregeanus, Chionochloa macra, Coleataenia prionitis, Danthonia californica , Digitaria exilis, Echinochloa oryzicola, Eragrostis tef, Eremitis sp., Isachne distichophylla, Hilaria cenchroides , Hickelia madagascariensis, Microlaena stipoides, Miscanthus sinensis, Neololeba atra, Olmeca reflexa, Oryza sativa, Otatea acuminata, Panicum miliaceum , Pariana campestris, Phragmites australis, Potamophila parviflora, Puelia olyriformis, Rhynchoryza subulata, Saccharum hybrid, Saccharum officinarum, Setaria viridis, Sorghum bicolor, Sorghum timorense, Sporobolus maritimus, Stipa purpurea, Triticum aestivum, Zoysia macrantha Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Application Examples

Application example

western blot using anti-RpoC antibodies

Stroma, thylakoid, nuclei and total protein from Arabidopsis thaliana and Zea mays were extracted with 50 mM HEPES-KOH pH 8, 330 mM sorbitol exaction buffer. Components were separated on 12% SDS-PAGE and blotted overnight to Nitrocellulose membrane using wet transfer. Blots were blocked with 5% non-fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5min with chemiluminescent detection reagent of extreme low femtogram range. Exposure time was 120 seconds.

Courtesy of Jie Shen and Dr. Alice Barkan, University of Oregon, USA

Additional Information
Additional information (application): There may be occur a cross reacting band at 100 kDa in maize samples
Background

Background:

RpoC1 (RNA polymerase beta' subunit (chloroplast)) is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

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Number of reviews: (2)

Wojciech Majeran | 2024-03-01

0 | 0

Used with Zea mays, protein load 5 ug total, clean signal on chloroplast and nucleoid fractions. Dilution 1:2000 works well in nucleoid enriched fractions, total chloroplast requires 1:1000 dilution

Reference yu Qing-bo, ph.d | 2019-04-25

0 | 0

It works well against Arabidopsis thylakoid protein.