RpoTm | DNA-directed mitochondrial RNA polymerase (RpoT1; mitochondrial NEP)
AS15 3078 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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1 : 2 000 with ECL (WB)
|Expected | apparent MW||
111.9 | 110 kDa
|Confirmed reactivity||Arabidopsis thaliana, Nicotiana tabcum, Spinacia oleracea, Solanum lycopersicum,
|Predicted reactivity||Arabis alpina, Amborella trichopoda, Beta vulgaris, Brassica napus, Brassica oleracea, Capsella rubella, Citrus sinensis, Chenopodium album, Coffea canephora, Cucumis sativus, Erythranthe guttata, Eucalyptus grandis, Eutrema salsugineum, Glycine soja, Gossypium raimondii, Medicago truncatula, Morus notabilis, Nicotiana tabacum, Phaseolus vulgaris, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Vitis vinifera|
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
Antibodies works on a total cell extract and purified mitochondria.
|Selected references||To be added when available, antibody released in December 2015|
2.5 ng (1), 5 ng (2), 10 ng (3) of recombinant Arabidopsis thaliana RPOTm and 40 µg of a total cell extract from Arabidopsis thaliana leaves (4) were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 1 to 5 seconds.
Band at 110 kDa represents fully matured protein.
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