RPS5 | ribosomal protein S5 (chloroplastic)

340 €

AS15 3075  | clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, H. vulgare, N. tabacum, S. lycopersicum, S. oleracea


19 st
Item No:
AS15 3075

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product information
Background Ribosomal protein S5, chloroplastic is a ribosomal protein from S5 family protein. It functions as a structural constituent of ribosome, RNA binding. It is involved in: response to cadmium ion, response to cold and translation.

Recombinant S5 protein from Spinacia oleracea, UniProt: Q9ST69

Host Rabbit
Clonality Polyclonal
Purity Serum
Quantity 100 ĩl

For reconstitution add 100 µl of sterile water.

Lyophilized antibody can be stored at -20 or -80°C.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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Secondary antibodies

Additional information
application information
Recommended dilution

1:2000 with ECL (WB)

Expected | apparent MW

27.8 | 27.5 kDa

Confirmed reactivity

Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Solanum lycopersicum (with higher load /well), Spinacia oleracea

Predicted reactivity Arthrospira platensis, Beta vulgaris, Glycine soja, Gossypium arboreum, Medicago truncatul, Morus notabilis, Oryza sativa, Phaseolus vulgaris, Riciunus communis, Theobroma cacao, Zea mays, Zostera marin, for reactivity on algae - please inquire
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references to be added when available, antibody released in December 2015

application example

western blot using anti-RPS5 antibodies

5 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary anti-S5 antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 15 seconds.

RPS5 antibodies will react with Solanum lycopersicum when load per well is increased to 10-15 µg/well.

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