RPS7 | ribosomal protein S7 (chloroplastic)

350 €

AS15 2877  | clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, H. vulgare, N. tabacum, S. lycopersicum, S. oleracea


15 st
Item No:
AS15 2877

Info: Product suggestions Add review
product information
Background Ribosomal protein S7, chloroplastic, is a protein that is a part of the small subunit of the ribosomal complex. It is one of the primary rRNA binding proteins.

full length, recombinant S7 protein from Arabidopsis thaliana, UniProt: P61841, TAIR: ATCG00900

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl

For reconstitution add 100 µl of sterile water.

Lyophilized antibody can be stored at -20 or -80°C.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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Secondary antibodies

Additional information
application information
Recommended dilution

1:3000 with standard ECL (WB)

Expected | apparent MW

17.4 kDa

Confirmed reactivity

Arabidopsis thaliana, Nicotiana tabacum,Hordeum vulgare, Spinacia oleracea

Predicted reactivity Brassica napus, Capsella rubella, Citrus sinensis, Cucumis sativus, Gossypium arboreum, Ricinus communis, Populus trichocarpa, Solanum lycopersicum, Solanum tuberosumVitis vinifera,
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references to be added when available, antibody released in May 2015

application example

western blot using anti-plant RPS7 antibodies

20 µg of total protein from Spinacia oleracea (1), Arabidopsis thaliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5) leafs were separated on 12 % SDS-PAGE  and blotted 1h to PVDF using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10 seconds.

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