IKP

Rubisco ELISA quantitation kit

Product no: AS15 2994

AS15 2994 | Reactivity: Rubisco protein

This set enables quantification of Rubisco using ELISA in samples from a range of various species.

1019
Add to cart
Delivery:  3-6 business days
  • Data sheet
  • Background | This ELISA immunoassay utilize the principle of competitive inhibition for quantitative determination of endogenous Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) in plant tissues.

    Rubisco antigen is pre-coated on the surface of the micro titer plate. Standards or samples are added to the plate wells including an antibody specific for Rubisco. The reaction of competitive inhibition is initiated between the pre-coated Rubisco and Rubisco in the samples with the antibody. A goat anti-rabbit IgG, HRP-conjugated, antibody is added followed by a substrate solution and the color develops which is inversely proportional to the amount of Rubisco in the sample. The intensity of the color is measured after reaction is is   stopped.


    This Rubisco ELISA kit contains the following reagents:
    Assay plate: 1x (96 wells)
    Standards: 2 x 500 µl
    Antibody: 1 x 60 µl (100 x concentrate)
    HRP-conjugate: 1 x 120 µl (100 x concentrate)
    Antibody Diluent: 1 x 10 ml
    HRP-conjugate Diluent: 1 x 20 ml
    Sample Diluent: 2 x 20 ml
    Sample Extraction Buffer: 1 x 20 ml (25 x concentrate)
    Washing Buffer: 1 x 20 ml (25 x concentrate)
    TMB Substrate: 1 x 10 ml (store in the dark)
    Stop Solution: 1 x 10 ml (contains acid, wear eye, hand, face and clothing protection)
    Adhesive Strip: 4x (for 96 wells)

    Agrisera Rubisco ELISA quantitation kit
    This kit is for research purpose only.

    ________________________________________________________________________________________

    Storage: Store at 2-8°C. Opened kit can be stored at 2-8°C for maximum of 1 month.

    Expiry date:
    Kit should not be used beyond expiry date.

    Detection range:
    3.12-800 µg/ml

    Specificity/Sensitivity:
    High specificity and sensitivity for plant Rubisco. No significant cross reactivity has been observed.


    Manual in Pdf

    Related products

    AS03 037 | RbcL | Rubisco large subunit, form I and form II (50 µl) 
    AS03 037A | anti-RbcL | Rubisco large subunit, form I and form II (50 µg affinity purified)
    AS03 037-HRP| anti-RbcL | Rubisco large subunit, form I and form II (40 µg, HRP-conjugated)

    AS15 2955
    | anti-RbcL II | Rubisco large subunit, form II (50 µl), rabbit antibody
    AS15 2955S | RbcL II | Rubisco form II positive control/quantitation standard

    AS01 017  | anti-RbcL | Rubisco large subunit, form I, chicken antibody
    AS01 017S | Rubisco protein standard for quantitative western blot or positive control

    AS09 409 | Rubisco quantitation kit

    AS07 218  | anti-Rubisco | 557 kDa hexadecamer, rabbit antibody to a whole protein

    AS07 259
    | anti-RbcS | Rubisco small subunit (SSU), rabbit antibody
    AS07 222
    | anti-RbcS | Rubisco small subunit (SSU) from pea, rabbit antibody

    None of anti-Rubisco antibodies listed above is included in this ELISA kit.

    matching secondary antibody
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS03 037 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: global antibody  and compartment marker for higher plants, lichens, algae, cyanobacteria, dinoflagellates, diatoms 

Benefits of using this antibody

319 €
Add to cart
Info
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

AS15 2955 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:  photosynthetic bacteria, archea, dinoflagellates

Benefits of using this antibody

319 €
Add to cart
Info
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
AS07 218 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: higher plants, lichens, algae
319 €
Add to cart
Info
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

AS01 017  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: [global antibody] for higher plants, algae, cyanobacteria  |  cellular [compartment marker] of plastid stroma

Benefits of using this antibody

416 €
Add to cart
Info
Close