Rubisco | 557 kDa hexadecamer

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000)

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS03 037| anti-RbcL (Rubisco large subunit) form I and II  rabbit antibody, most sold Agrisera Rubisco antibody

AS01 017S | Rubisco protein standard for quantitation or positive control

AS01 017 | anti-RbcL (Rubisco large subunit) form I hen antibody

AS07 259 | anti-RbcS | Rubisco small subunit (SSU)

AS07 222 | anti-RbcS | Rubisco small subunit (SSU) from pea

to be added when available