Plant/Algal cell antibodies / Photosynthesis / RUBISCO

product information

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000)

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS03 037| anti-RbcL (Rubisco large subunit) form I and II  rabbit antibody, most sold Agrisera Rubisco antibody

AS01 017S | Rubisco protein standard for quantitation or positive control

AS01 017 | anti-RbcL (Rubisco large subunit) form I hen antibody

AS07 259 | anti-RbcS | Rubisco small subunit (SSU)

AS07 222 | anti-RbcS | Rubisco small subunit (SSU) from pea

to be added when available

application information

1:10 000 - 1:20 000 on 0.5-10 ug total cellular protein/lane and standard ECL (WB)

53-55 | 53-55 kDa

dicots including: Arabidopsis thaliana, Glycine max, Manihot esculenta Crantz Pisum sativum, Solanum tuberosum, Spinacia oleracea; monocots including: Zea mays; trees: Populus sp.moss: Physcomitrella patens, algae: Chlamydomonas reinhardtii, Synechococcus sp. PCC7942, Synechocystis sp. PCC 6803

dicots including: Begonia sp., Daucus carota and others

no confirmed exceptions from predicted reactivity known in the moment

RbcS subunit is not detected by this antibody

Kissen et al. (2012). Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana. Plant Mol. Bio. - ahead of print.

Li et al. (2010). Proteome characterization of cassava (Manihot esculenta Crantz) somatic embryos, plantlets and tuberous roots. Proteome Sci. 2010 Feb 27;8:10.